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”, la transferencia puede ser realizada por difusión molecular, por un flujo de buffer inducido por la succión (microfiltración) o acción capilar, el método utilizado de manera más frecuente es la electrotransferencia.

La es un método simple pero muy ineficiente para aplicar a la mayoría de las transferencias, exepto para las transferencias de geles de agarosa.

Generalmente las proteínas separadas en geles de poliacrlilamida se procesan por electroblotting, que utiliza la furza motriz de un campo eléctrico para que las proteínas eluyan desde el gel para inmovilizarlos en una matriz.

Este método es rápido, eficiente y mantiene una alta resolución del patrón de bandas de las proteínas separadas por electroforesis.

Existen dos principales configuraciones para este método: Como puede notarse la transferencia Semiseca utiliza menos glicina y permite una transferencia más rápida pero no es muy efectiva para proteínas mayores a 120 KDa.

En la siguiente tabla podemos ver una comparación entre ambos sistemas Eficiencia variable de transferencia para proteínas mayores a 120 KDa (puede ser mejorado con un sistema de buffer discontinuo); las proteínas de bajo peso molecular pueden ser transferidas a través de la membrana., una concentración elevada de SDS, puede bloquear la unión de las proteínas a las membranas, pero el 0.01% facilita la transferencia, especialmente de proteínas grandes.

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